diff --git a/spring-tx/src/main/java/org/springframework/transaction/support/AbstractPlatformTransactionManager.java b/spring-tx/src/main/java/org/springframework/transaction/support/AbstractPlatformTransactionManager.java
index 894580699ec..39ac0bbfea1 100644
--- a/spring-tx/src/main/java/org/springframework/transaction/support/AbstractPlatformTransactionManager.java
+++ b/spring-tx/src/main/java/org/springframework/transaction/support/AbstractPlatformTransactionManager.java
@@ -416,7 +416,8 @@ public abstract class AbstractPlatformTransactionManager
 						"isolation level will effectively be ignored: " + def);
 			}
 			boolean newSynchronization = (getTransactionSynchronization() == SYNCHRONIZATION_ALWAYS);
-			return prepareTransactionStatus(def, null, true, newSynchronization, debugEnabled, null);
+			SuspendedResourcesHolder suspendedResources = suspend(null);
+			return prepareTransactionStatus(def, null, true, newSynchronization, debugEnabled, suspendedResources);
 		}
 	}
 
diff --git a/spring-tx/src/test/java/org/springframework/transaction/support/TransactionSupportTests.java b/spring-tx/src/test/java/org/springframework/transaction/support/TransactionSupportTests.java
index da661fdd9c7..4a7dd6ebf59 100644
--- a/spring-tx/src/test/java/org/springframework/transaction/support/TransactionSupportTests.java
+++ b/spring-tx/src/test/java/org/springframework/transaction/support/TransactionSupportTests.java
@@ -16,34 +16,23 @@
 
 package org.springframework.transaction.support;
 
-import java.lang.reflect.Field;
-import java.util.Arrays;
-import java.util.HashSet;
-import java.util.Set;
-import java.util.stream.Stream;
-
 import org.junit.jupiter.api.AfterEach;
 import org.junit.jupiter.api.Nested;
 import org.junit.jupiter.api.Test;
-
-import org.springframework.transaction.IllegalTransactionStateException;
-import org.springframework.transaction.PlatformTransactionManager;
-import org.springframework.transaction.TransactionDefinition;
-import org.springframework.transaction.TransactionStatus;
-import org.springframework.transaction.TransactionSystemException;
+import org.springframework.transaction.*;
 import org.springframework.transaction.testfixture.MockCallbackPreferringTransactionManager;
 import org.springframework.transaction.testfixture.TestTransactionExecutionListener;
 import org.springframework.util.ReflectionUtils;
 
-import static org.assertj.core.api.Assertions.assertThat;
-import static org.assertj.core.api.Assertions.assertThatExceptionOfType;
-import static org.assertj.core.api.Assertions.assertThatIllegalArgumentException;
-import static org.assertj.core.api.Assertions.assertThatRuntimeException;
-import static org.springframework.transaction.TransactionDefinition.ISOLATION_REPEATABLE_READ;
-import static org.springframework.transaction.TransactionDefinition.ISOLATION_SERIALIZABLE;
-import static org.springframework.transaction.TransactionDefinition.PROPAGATION_MANDATORY;
-import static org.springframework.transaction.TransactionDefinition.PROPAGATION_REQUIRED;
-import static org.springframework.transaction.TransactionDefinition.PROPAGATION_SUPPORTS;
+import java.lang.reflect.Field;
+import java.util.Arrays;
+import java.util.HashSet;
+import java.util.Set;
+import java.util.stream.Stream;
+
+import static org.assertj.core.api.Assertions.*;
+import static org.springframework.transaction.TransactionDefinition.*;
+import static org.springframework.transaction.support.AbstractPlatformTransactionManager.SYNCHRONIZATION_ALWAYS;
 import static org.springframework.transaction.support.AbstractPlatformTransactionManager.SYNCHRONIZATION_ON_ACTUAL_TRANSACTION;
 import static org.springframework.transaction.support.DefaultTransactionDefinition.PREFIX_ISOLATION;
 import static org.springframework.transaction.support.DefaultTransactionDefinition.PREFIX_PROPAGATION;
@@ -78,6 +67,58 @@ class TransactionSupportTests {
 				.isThrownBy(() -> tm.getTransaction(new DefaultTransactionDefinition(PROPAGATION_MANDATORY)));
 	}
 
+	@Test
+	void noExistingTransactionWithExistingAnotherTransactionManager() {
+		AbstractPlatformTransactionManager tm1 = new TestTransactionManager(false, true);
+		tm1.setTransactionSynchronization(SYNCHRONIZATION_ALWAYS);
+
+		DefaultTransactionDefinition txDef1 =
+				new DefaultTransactionDefinition(PROPAGATION_REQUIRED);
+		txDef1.setName("tx1");
+		txDef1.setReadOnly(false);
+		txDef1.setIsolationLevel(ISOLATION_READ_COMMITTED);
+
+		DefaultTransactionStatus txStatus1 = (DefaultTransactionStatus)tm1.getTransaction(txDef1);
+
+		// assert for txStatus1 and TransactionSynchronizationManager property
+		assertThat(txStatus1.hasTransaction()).as("Must have transaction").isTrue();
+		assertThat(txStatus1.isNewTransaction()).as("Must be new transaction").isTrue();
+		assertThat(TransactionSynchronizationManager.isCurrentTransactionReadOnly())
+				.as("Transaction1 Must be readOnly false")
+				.isEqualTo(txStatus1.isReadOnly());
+		assertThat(TransactionSynchronizationManager.getCurrentTransactionName())
+				.as("TransactionSynchronizationManager have correct transaction name")
+						.isEqualTo(txStatus1.getTransactionName());
+		assertThat(TransactionSynchronizationManager.getCurrentTransactionIsolationLevel())
+				.as("isolation level must be default").isNull();
+		assertThat(TransactionSynchronizationManager.isSynchronizationActive()).isTrue();
+
+		// Setting another trnasaction manager
+		AbstractPlatformTransactionManager tm2 = new TestTransactionManager(false, true);
+		tm2.setTransactionSynchronization(SYNCHRONIZATION_ALWAYS);
+
+		// Opening a new transaction before `transaction 1` commits.
+		DefaultTransactionDefinition txDef2 =
+				new DefaultTransactionDefinition(PROPAGATION_SUPPORTS);
+		txDef2.setReadOnly(true);
+		txDef2.setIsolationLevel(ISOLATION_REPEATABLE_READ);
+		txDef2.setName("tx2");
+
+		// assert for txStatus1 and TransactionSynchronizationManager property
+		DefaultTransactionStatus txStatus2 = (DefaultTransactionStatus)
+				tm2.getTransaction(txDef2);
+		assertThat(TransactionSynchronizationManager.isActualTransactionActive()).as("Must not have transaction")
+				.isEqualTo(txStatus2.hasTransaction());
+		assertThat(TransactionSynchronizationManager.isCurrentTransactionReadOnly()).as("Must be readOnly true")
+				.isEqualTo(txStatus2.isReadOnly());
+		assertThat(TransactionSynchronizationManager.getCurrentTransactionIsolationLevel())
+				.as("isolation level must be Repeatable Read")
+				.isEqualTo(ISOLATION_REPEATABLE_READ);
+		assertThat(TransactionSynchronizationManager.isSynchronizationActive()).isTrue();
+
+		TransactionSynchronizationManager.clearSynchronization();
+	}
+
 	@Test
 	void existingTransaction() {
 		PlatformTransactionManager tm = new TestTransactionManager(true, true);