diff --git a/spring-r2dbc/src/main/java/org/springframework/r2dbc/connection/R2dbcTransactionManager.java b/spring-r2dbc/src/main/java/org/springframework/r2dbc/connection/R2dbcTransactionManager.java
index 7aea0c270a8..fb6d57a4efa 100644
--- a/spring-r2dbc/src/main/java/org/springframework/r2dbc/connection/R2dbcTransactionManager.java
+++ b/spring-r2dbc/src/main/java/org/springframework/r2dbc/connection/R2dbcTransactionManager.java
@@ -80,6 +80,7 @@ import org.springframework.util.Assert;
  * transaction definitions for vendor-specific attributes.
  *
  * @author Mark Paluch
+ * @author Juergen Hoeller
  * @since 5.3
  * @see ConnectionFactoryUtils#getConnection(ConnectionFactory)
  * @see ConnectionFactoryUtils#releaseConnection
@@ -149,7 +150,7 @@ public class R2dbcTransactionManager extends AbstractReactiveTransactionManager
 	 * transactional connection: "SET TRANSACTION READ ONLY" as understood by Oracle,
 	 * MySQL and Postgres.
 	 * <p>The exact treatment, including any SQL statement executed on the connection,
-	 * can be customized through through {@link #prepareTransactionalConnection}.
+	 * can be customized through {@link #prepareTransactionalConnection}.
 	 * @see #prepareTransactionalConnection
 	 */
 	public void setEnforceReadOnly(boolean enforceReadOnly) {
@@ -209,8 +210,9 @@ public class R2dbcTransactionManager extends AbstractReactiveTransactionManager
 				connectionMono = Mono.just(txObject.getConnectionHolder().getConnection());
 			}
 
-			return connectionMono.flatMap(con -> prepareTransactionalConnection(con, definition, transaction)
+			return connectionMono.flatMap(con -> switchAutoCommitIfNecessary(con, transaction)
 					.then(Mono.from(doBegin(definition, con)))
+					.then(prepareTransactionalConnection(con, definition))
 					.doOnSuccess(v -> {
 						txObject.getConnectionHolder().setTransactionActive(true);
 						Duration timeout = determineTimeout(definition);
@@ -375,6 +377,24 @@ public class R2dbcTransactionManager extends AbstractReactiveTransactionManager
 		});
 	}
 
+	private Mono<Void> switchAutoCommitIfNecessary(Connection con, Object transaction) {
+		ConnectionFactoryTransactionObject txObject = (ConnectionFactoryTransactionObject) transaction;
+		Mono<Void> prepare = Mono.empty();
+
+		// Switch to manual commit if necessary. This is very expensive in some R2DBC drivers,
+		// so we don't want to do it unnecessarily (for example if we've explicitly
+		// configured the connection pool to set it already).
+		if (con.isAutoCommit()) {
+			txObject.setMustRestoreAutoCommit(true);
+			if (logger.isDebugEnabled()) {
+				logger.debug("Switching R2DBC Connection [" + con + "] to manual commit");
+			}
+			prepare = prepare.then(Mono.from(con.setAutoCommit(false)));
+		}
+
+		return prepare;
+	}
+
 	/**
 	 * Prepare the transactional {@link Connection} right after transaction begin.
 	 * <p>The default implementation executes a "SET TRANSACTION READ ONLY" statement if the
@@ -385,33 +405,16 @@ public class R2dbcTransactionManager extends AbstractReactiveTransactionManager
 	 * override this method accordingly.
 	 * @param con the transactional R2DBC Connection
 	 * @param definition the current transaction definition
-	 * @param transaction the transaction object
+	 * @since 5.3.22
 	 * @see #setEnforceReadOnly
 	 */
-	protected Mono<Void> prepareTransactionalConnection(
-			Connection con, TransactionDefinition definition, Object transaction) {
-
-		ConnectionFactoryTransactionObject txObject = (ConnectionFactoryTransactionObject) transaction;
-
+	protected Mono<Void> prepareTransactionalConnection(Connection con, TransactionDefinition definition) {
 		Mono<Void> prepare = Mono.empty();
-
 		if (isEnforceReadOnly() && definition.isReadOnly()) {
 			prepare = Mono.from(con.createStatement("SET TRANSACTION READ ONLY").execute())
 					.flatMapMany(Result::getRowsUpdated)
 					.then();
 		}
-
-		// Switch to manual commit if necessary. This is very expensive in some R2DBC drivers,
-		// so we don't want to do it unnecessarily (for example if we've explicitly
-		// configured the connection pool to set it already).
-		if (con.isAutoCommit()) {
-			txObject.setMustRestoreAutoCommit(true);
-			if (logger.isDebugEnabled()) {
-				logger.debug("Switching R2DBC Connection [" + con + "] to manual commit");
-			}
-			prepare = prepare.then(Mono.from(con.setAutoCommit(false)));
-		}
-
 		return prepare;
 	}
 
@@ -452,21 +455,20 @@ public class R2dbcTransactionManager extends AbstractReactiveTransactionManager
 	 * to R2DBC drivers when starting a transaction.
 	 */
 	private record ExtendedTransactionDefinition(@Nullable String transactionName,
-												boolean readOnly,
-												@Nullable IsolationLevel isolationLevel,
-												Duration lockWaitTimeout) implements io.r2dbc.spi.TransactionDefinition {
+			boolean readOnly, @Nullable IsolationLevel isolationLevel, Duration lockWaitTimeout)
+			implements io.r2dbc.spi.TransactionDefinition {
 
 		private ExtendedTransactionDefinition(@Nullable String transactionName, boolean readOnly,
 				@Nullable IsolationLevel isolationLevel, Duration lockWaitTimeout) {
+
 			this.transactionName = transactionName;
 			this.readOnly = readOnly;
 			this.isolationLevel = isolationLevel;
 			this.lockWaitTimeout = lockWaitTimeout;
 		}
 
-
-		@Override
 		@SuppressWarnings("unchecked")
+		@Override
 		public <T> T getAttribute(Option<T> option) {
 			return (T) doGetValue(option);
 		}